Study on tissue culture and plant regeneration of Nervilia fordii / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>Tissue culture of Nervilia fordii to get its regeneration.</p><p><b>METHOD</b>Effects on indusement of rhizoma and plant regeneration of different implants, density of hormones, additives were studied.</p><p><b>RESULT</b>The best implant was conn. Effect of 6-BA 2 mg x L(-1) were better than 6-BA 1 on rhizoma reducing. The coconut juice and active carbon could increase the growth of rhizoma.</p><p><b>CONCLUSION</b>Bud could be induced on 1/2MS + 6-BA 2 mg x L(-1) by inoculating corm on culture mediem, and could grow lots of rhizoma after inoculating on the culture mediem containing 10% coconut juice and 1 per thousand active carbon. The white rhizoma could be induced to corms and regeneration plants on 1/2MS + 1 per thousand active carbon. The green rhizoma could be induced directerly to regeneration plants on 1/2MS + 6-BA 2 + NAA 2.</p>