A study on the hairy root culture and antitumor alkaloids production of Catharanthus roseus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 741-755, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-358120
ABSTRACT
<p><b>OBJECTIVE</b>To establish transformation system and obtain alkaloids from the hairy root of Catharanthus roseus.</p><p><b>METHOD</b>Hairy roots were obtained by infecting the different explants of C. roseus. Culture conditions of hairy root were optimized.</p><p><b>RESULT</b>The best transformation condition was leaf infected by two-day's pre-culture and two-day's co-culture and additional A(S) (hydroxyacetosyringone) 100 mg x L(-1). The inducing rate of hairy root was up to 86.25%. The best condition of hairy root culture was MS medium with sucrose as carbon material and lactalbumin as nitron material. The analysis result showed that the contents of total alkaloids in hairy roots were higher than explants and calli.</p><p><b>CONCLUSION</b>Hairy root of C. roseus will be useful for the production of active components in C. roseus.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Rhizobium
/
Sacarosa
/
Vinblastina
/
Vincristina
/
Química
/
Raíces de Plantas
/
Medios de Cultivo
/
Catharanthus
/
Técnicas de Cultivo de Tejidos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2005
Tipo del documento:
Artículo
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