ITS sequence identification of Radix Bupleuri / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 732-734, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-358123
ABSTRACT
<p><b>OBJECTIVE</b>To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer (ITS) sequences of five kinds of Radix Bupleuri in common use.</p><p><b>METHOD</b>Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification.</p><p><b>RESULT</b>The length of ITS1 and ITS2 sequence was 214-220 bp and 230-231 bp respectively. There were distinct variation sites between the ITS sequences of the five kinds of Radix Bupleuri.</p><p><b>CONCLUSION</b>ITS sequence may be the evidence for the molecular authentication of Radix Bupleuri.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Plantas Medicinales
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Clasificación
/
Raíces de Plantas
/
ADN de Plantas
/
ADN Espaciador Ribosómico
/
Bupleurum
/
Cuminum
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2005
Tipo del documento:
Artículo
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