Changes of nuclear factor and inflammatory chemotactic factors in brain of patients with Alzheimer's disease / 中华病理学杂志
Chinese Journal of Pathology
; (12): 585-589, 2011.
Article
en Zh
| WPRIM
| ID: wpr-358290
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the changes of nuclear factor (NF-)κBp65 and inflammatory chemotactic factors including monocyte chemoattractant protein 1 (MCP-1/CCL-2), macrophage inflammatory protein 1α (MIP-1α/CCL-3), glial fibrillary acidic protein (GFAP) in brains of the patients with Alzheimer's disease (AD) and reveal the correlation of these factors.</p><p><b>METHODS</b>Ten patients with AD and 8 age-matched control subjects were selected in the study. Immunohistochemistry was performed to determine the protein expression of NF-κBp65, MCP-1, MIP-1α and GFAP. Double-immunohistochemistry was used to detect the expression of GFAP and β-amyloid peptide 1-42 (Aβ(1-42)) in the hippocampus, temporal and frontal cortices.</p><p><b>RESULTS</b>As compared to age-matched controls (the numbers of the positively stained neuronal cells: 0.31 ± 0.20, 0.25 ± 0.20 and 0.25 ± 0.20, respectively), the immunoreactivities of NF-κBp65 in the hippocampus and the temporal and frontal cortices (numbers of the positively stained cells: 3.6 ± 1.5, 2.2 ± 1.2 and 2.2 ± 1.2, respectively) were significantly increased in AD brains. The levels of MCP-1 and MIP-1α in the hippocampus, and the temporal and frontal cortices (numbers of the positively stained neuronal cells: 8.0 ± 1.3, 8.8 ± 1.0, 9.3 ± 1.4, respectively;and 8.1 ± 1.5, 12.5 ± 1.1, 6.4 ± 1.1, respectively) with AD were significantly higher than those of controls (the numbers of the positive neuronal cells: 4.5 ± 0.9, 4.5 ± 0.6, 4.0 ± 1.8, respectively; and 5.0 ± 1.9, 6.3 ± 2.2, 3.8 ± 1.5, respectively). An increased number of glial cells stained with GFAP were observed to extensively distribute around the senile plaques in AD brains. There were significant correlations between NF-κBp65 and these inflammatory chemotactic factors in AD brains.</p><p><b>CONCLUSION</b>Correlative expressions of NF and inflammatory chemotactic factors were found in the brains of AD patients, through a mechanism that may involve the inflammatory response induced by Aβ in the processing of AD.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
/
Lóbulo Temporal
/
Encéfalo
/
Inmunohistoquímica
/
Neuroglía
/
Quimiocina CCL2
/
Placa Amiloide
/
Factor de Transcripción ReIA
/
Quimiocina CCL3
/
Enfermedad de Alzheimer
Límite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Año:
2011
Tipo del documento:
Article