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Detection of multidrug-resistance proteins with protein array chips / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 528-530, 2005.
Artículo en Chino | WPRIM | ID: wpr-358580
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the use of protein array chips in detection of multidrug-resistance proteins.</p><p><b>METHODS</b>Human erythroleukemic cell line K562 and its doxorubicin-resistant counterpart K562/A02 were used in the study. Monoclonal antibodies against P-glycoprotein (P-gP), multidrug resistance-associated protein (MRP1) and breast cancer resistance protein (BCRP) were immobilized onto agarose film-coated glass. The antibody-cell binding was assessed by capturing K562 and K562/A02 cells. The protein array was observed under a microscope and the image was captured with a CCD camera. The expression levels of the three proteins were also measured by flow cytometry (FCM).</p><p><b>RESULTS</b>The expression of P-gP and BCRP in K562 was very low. However, MRP1 expression was high. P-gP and MRP1 were highly expressed in K562/A02, while the expression of BCRP was low. FCM results showed that the expression rate of P-gP, MRP1 and BCRP in K562 cells was 5.98% +/- 2.19%, 95.80% +/- 3.98%, 1.03% +/- 0.45%, respectively, while that in K562/A02 cells was 92.67% +/- 1.80%, 97.18% +/- 1.02%, 3.98% +/- 0.37%, respectively. The results of protein array method are consistent with those of FCM (P > 0.05).</p><p><b>CONCLUSION</b>It is feasible to develop a new protein array technique and to provide a novel method for multi-drug resistant cell detection, with a high throughput, high specificity, simple procedure and low cost.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Miembro 1 de la Subfamilia B de Casetes de Unión a ATP / Resistencia a Múltiples Medicamentos / Transportadoras de Casetes de Unión a ATP / Resistencia a Antineoplásicos / Células K562 / Proteínas Asociadas a Resistencia a Múltiples Medicamentos / Análisis por Matrices de Proteínas / Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2 / Genética / Proteínas de Neoplasias Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Chinese Journal of Oncology Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Miembro 1 de la Subfamilia B de Casetes de Unión a ATP / Resistencia a Múltiples Medicamentos / Transportadoras de Casetes de Unión a ATP / Resistencia a Antineoplásicos / Células K562 / Proteínas Asociadas a Resistencia a Múltiples Medicamentos / Análisis por Matrices de Proteínas / Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2 / Genética / Proteínas de Neoplasias Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Chinese Journal of Oncology Año: 2005 Tipo del documento: Artículo