Comparison of membrane current of vascular smooth muscle cells in brain artery of spontaneously hypertensive rats and Wistar rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 25-28, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-358688
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the difference in membrane current of vascular smooth muscle cells (VSMCs) in brain artery (BA) of spontaneously hypertensive rats (SHR) and Wistar rats.</p><p><b>METHODS</b>We compared the properties of spontaneous transient outward K+ currents (STOCs), the density and composition of current of VSMCs in BA of SHR and Wistar rats by whole-cell patch clamp technique.</p><p><b>RESULTS</b>(1) When the command voltage was 0, + 20, + 40 and + 60 mV respectively, the current densities of VSMCs in BA of SHR and Wistar rats were significant different (P < 0.01). (2) The whole-cell current of VSMCs was partly inhibited by 1 mmol/L4-AP (voltage-gated K+ channel blocker) or 1 mmol/L TEA (big conductance Ca(2+)-activated K+ channel blocker) respectively. (3) The frequency and amplitude of STOCs in SHR were faster and bigger than those in Wistar rats. 1 mmol/L TEA almostly inhibited the STOCs, but not by 4-AP.</p><p><b>CONCLUSION</b>These results suggest that the current densities of VSMCs in BA of SHR and Wistar rats are significant different, the outward current of VSMCs in BA of SHR and Wistar rats are composed by Kv and BK(Ca). SHR express more STOCs mediated by BK(Ca), than Wistar rats.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Ratas Endogámicas SHR
/
Arterias Cerebrales
/
Ratas Wistar
/
Técnicas de Placa-Clamp
/
Biología Celular
/
Canales de Potasio Calcio-Activados
/
Canales de Potasio con Entrada de Voltaje
/
Miocitos del Músculo Liso
/
Potenciales de la Membrana
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2013
Tipo del documento:
Artículo
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