Construction and expression of hTNF-alpha fusion protein mediated by MMP1 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 534-537, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-359230
ABSTRACT
This paper is aimed to present a research on fusion protein of human tumor necrosis factor-alpha (hTNF-alpha), matrix metalloproteinase 1 (MMP1), and foldon sequence using the methord of gene engineering. We transformed the recombinant plasmid, which contains the DNA sequences of hTNF-alpha, MMP1, and foldon sequence, into Rosetta2, and successfully induced the fusion protein to express under given conditions by isopropyl beta-D-1-Thiogalactopyranoside (IPTG). Then we purified the expression product through a glutathione S-transferase (GST) resin and collected the interested protein. This research may lay the groundwork for scientific research and clinical application.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes de Fusión
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Factor de Necrosis Tumoral alfa
/
Metaloproteinasa 1 de la Matriz
/
Escherichia coli
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2011
Tipo del documento:
Artículo
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