Proteomics study of the serum biomarkers in tongue cancer / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.</p><p><b>METHODS</b>The tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).</p><p><b>RESULTS</b>Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice.</p><p><b>CONCLUSIONS</b>The squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.</p>