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Epigenetic Changes of Serotonin Transporter in the Patients with Alcohol Dependence: Methylation of an Serotonin Transporter Promoter CpG Island
Psychiatry Investigation ; : 130-133, 2011.
Artículo en Inglés | WPRIM | ID: wpr-35977
ABSTRACT

OBJECTIVE:

Psychiatric disorders such as depression, anxiety and alcohol dependence are associated with serotonin metabolism. We assessed the methylation level of the serotonin transporter (5-HTT) promoter region in control and alcohol dependent patients.

METHODS:

Twenty seven male patients who met the Diagnostic and Statistical Manual of Mental Disorder IV (DSM-IV) criteria for alcohol dependence were compared with fifteen controls. Polymerase chain reaction (PCR) assays of bisulfate-modified DNA were designed to amplify a part of the CpG island in the 5HTT gene. Pyrosequencing was performed and the methylation level at seven CpG island sites was measured.

RESULTS:

We found no differences in the methylation patterns of the serotonin transporter linked promoter region (5-HTTLPR) between alcohol-dependent and control subjects.

CONCLUSION:

Our negative finding may be because 5-HTT epigenetic variation may not affect the expression for 5-HTT or there may be other methylation site critical for its expression. To find out more conclusive result, repeating the study in more methylation sites with a larger number of samples in a well-controlled setting is needed.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ansiedad / ADN / Serotonina / Reacción en Cadena de la Polimerasa / Regiones Promotoras Genéticas / Islas de CpG / Depresión / Alcoholismo / Proteínas de Transporte de Serotonina en la Membrana Plasmática / Epigenómica Tipo de estudio: Estudio diagnóstico / Guía de Práctica Clínica Límite: Humanos / Masculino Idioma: Inglés Revista: Psychiatry Investigation Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ansiedad / ADN / Serotonina / Reacción en Cadena de la Polimerasa / Regiones Promotoras Genéticas / Islas de CpG / Depresión / Alcoholismo / Proteínas de Transporte de Serotonina en la Membrana Plasmática / Epigenómica Tipo de estudio: Estudio diagnóstico / Guía de Práctica Clínica Límite: Humanos / Masculino Idioma: Inglés Revista: Psychiatry Investigation Año: 2011 Tipo del documento: Artículo