Cyclooxygenase-2 expression is dependent upon epidermal growth factor receptor expression or activation in androgen independent prostate cancer / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 758-764, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-359913
ABSTRACT
<p><b>AIM</b>To investigate the expression of cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) and the possible mechanism in the development in androgen independent prostate cancer (AIPC).</p><p><b>METHODS</b>Immunohistochemistry was performed on paraffin-embedded sections with goat polyclonal against COX-2 and mouse monoclonal antibody against EGFR in 30 AIPC and 18 androgen dependent prostate cancer (ADPC) specimens. The effect of epidermal growth factor (EGF) treatments on the expression of COX-2 and signal pathway in PC-3 and DU-145 cells was studied using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. ELISA was used to measure prostaglandin E2 (PGE2) levels in the media of PC-3 and DU-145 incubated with EGF for 24 h.</p><p><b>RESULTS</b>COX-2 was positively expressed in AIPC and ADPC, which were predominantly in endochylema of prostate cancer (PCa) cells. Intense staining was seen in AIPC (80%) and in ADPC (55.5%), but there was no significant association between the two groups. EGFR expression was also positive in the two groups (61.8% in ADPC and 90% in AIPC, P < 0.01). A significant association was found between EGFR expression and a higher Gleason score (P < 0.05) or tumor stage (P < 0.05). The expression of PGE2 was increased in PC-3 and DU-145 cells after being incubated with EGF. Both p38MAPK and PI-3K pathway were involved in the PC-3 cell COX-2 upregulation course. In DU-145, only p38MAPK pathway was associated with COX-2 upregulation.</p><p><b>CONCLUSION</b>EGFR activation induces COX-2 expression through PI-3K and/or p38MAPK pathways. COX-2 and EGFR inhibitors might have a cooperative anti-tumor effect in PCa.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Pronóstico
/
Neoplasias de la Próstata
/
Ensayo de Inmunoadsorción Enzimática
/
Inmunohistoquímica
/
Transducción de Señal
/
Regulación Enzimológica de la Expresión Génica
/
Regulación Neoplásica de la Expresión Génica
/
Medio de Cultivo Libre de Suero
/
Fosfatidilinositol 3-Quinasas
Tipo de estudio:
Estudio pronóstico
Límite:
Anciano
/
Aged80
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2008
Tipo del documento:
Artículo
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