Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 667-674, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-359923
ABSTRACT
<p><b>AIM</b>To investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H(2)O(2)-induced oxidative stress in cultured mouse TM3 Leydig cells.</p><p><b>METHODS</b>We carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells.</p><p><b>RESULTS</b>MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 micromol H(2)O(2), and treatment of the cells with 250 microg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and CAT activity (74.6 units of CAT/mg protein, P < 0.001).</p><p><b>CONCLUSION</b>These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H(2)O(2)-induced oxidative stress.</p>
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Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Superóxido Dismutasa
/
Testosterona
/
ARN Mensajero
/
Extractos Vegetales
/
Peroxidación de Lípido
/
Catalasa
/
Supervivencia Celular
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Células Cultivadas
/
Estrés Oxidativo
Límite:
Animales
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2008
Tipo del documento:
Artículo
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