Tumor Growth, Local Invasion, Micrometastasis, and Lymph Node Metastasis of Oral Squamous Cell Carcinoma Visualized in Live Tissue by Green Fluorescent Protein Expression / Oral Science International
Oral Science International
;
: 45-53, 2005.
Artículo
en Inglés
| WPRIM
| ID: wpr-362732
ABSTRACT
We report the <i>in vitro</i> establishment of a highly stable green fluorescent protein (GFP) -expressing transfectant of a highly-invasive human tongue squamous cell carcinoma (HTSCC) cell line, SAS-H1. The fluorescent cells permitted the visualization of tumor growth, local invasion, micrometastasis and cervical lymph node metastasis after submucosal injection into the tongues of nude mice. SAS-H1 cells were transfected with the pEGFP-N1 expression vector containing the GFP and neomycin resistance genes. Stable SAS-H1 clones expressing high levels of GFP were selected stepwise <i>in vitro</i> in levels of geneticin (G418) of up to 3,500 μg/ml. Subsequent early stages of local invasion and micrometastasis were visualized by GFP fluorescence in a primary tumor of the tongue. Furthermore, lymph node metastasis was confirmed for all of the orthotopic transplants in mice. However, no distant metastases, including those of lung and liver, were observed. Thus, this model should be useful for studying the metastatic process and for evaluating anti-metastasis agents in pre-clinical trials.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Inglés
Revista:
Oral Science International
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS