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Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences
The Korean Journal of Parasitology ; : 109-112, 2016.
Artículo en Inglés | WPRIM | ID: wpr-36473
ABSTRACT
We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, 180 µm in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and 760-820 µm long and 320-450 µm wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Trematodos / ARN Ribosómico 18S / ADN Espaciador Ribosómico / Metacercarias Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Trematodos / ARN Ribosómico 18S / ADN Espaciador Ribosómico / Metacercarias Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2016 Tipo del documento: Artículo