Effect of F1KN preparation extracted from lonicera dasystyla flower on transformation of human lymphocytes

ABSTRACT

Materials and methods: The flowers of Lonicera dasystyla were collected at the suburbs of Ha Noi city. The total flavonoid extract of the flowers was chromatographed to obtain the fraction F1KN, which was submitted to the examination for the stimulatory effect on the blast transformation of human lymphocytes. Results: At the dose of 30 mcg/ml in PBS, F1KN was proved to have a good effect. After 72h in vitro culture, the 30 mcg/ml dose increased the potential transformation of human lymphocytes by about 7 times compared with the control samples (1600 versus 230 pulses/min, respectively), while in the same condition, phytohemagglutinin (PHA) at the dose of 10 mcg/ml, increased the redioactivity by about 13000 pulses/min.