Food and Drug Interactions: Effect of <i>Acanthopanax senticosus</i> Harms on CYP3A4 and CYP2C9 Activities (Part 3) / 日本補完代替医療学会誌

ABSTRACT

<b>Objective:</b> By using human liver microsomes (HLM), we analyzed the effects of 14 known components of <i>A.senticosus</i> Harms on the activities of CYP2C9 and CYP3A4.<br> <b>Methods and Results:</b> Sesamin and quercetin inhibited both enzyme activities, whereas quercitrin strongly inhibited CYP3A4 activity. The 50% inhibitory concentrations (IC₅₀s) of sesamin and quercetin on CYP2C9 activity were approximately 124- and 59-fold higher and the IC₅₀s of sesamin, quercetin, and quercitrin on CYP3A4 activity were approximately 427-, 135-, and 22-fold higher than that of <i>A. senticosus</i> Harms extract (ASE), respectively. All these components inhibited both CYP3A4 and CYP2C9 in a non-competitive manner. However, these components are present in small amounts in ASE.<br> <b>Conclusion:</b> Therefore, the food-drug interactions caused by <i>A. senticosus </i>Harms are presumed to be due to the additive or synergistic interaction of these components or the other existing components, including their metabolites.<br>