Expression of TRH Receptor Gene in GH-Secreting Piruitary Adenomas / 대한내분비학회지
Journal of Korean Society of Endocrinology
; : 349-356, 1997.
Article
en Ko
| WPRIM
| ID: wpr-37801
Biblioteca responsable:
WPRO
ABSTRACT
Background: To test the hypothesis that Galphas gene mutation may suppress the expression of TRH-R gene, we investigated whether hTRH-R gene expression is lower in human GH-secreting pituitary adenomas with Galphas mutation than in tumors without the mutation. Method: TRH-induced paradoxical response of GH was observed in 8 acromegalic patients. The mutation of gene was identified by direct sequencing of the genomic DNA prepared from GH-secreting pituitary adenomas. The expression of hTRHT mRNA was quantitated by RT-PCR. Results: The transcript of hTRH-R gene was detected in 6 of 8(75%) tumors. Three of these(50%) showed the paradoxical GH response to TRH and the other three patients did not show the response. The relative expression of hTRH-R mRNA in the tumors from patients with the paradoxical response of GH to TRH did not differ from that in the tumors from patients without the paradoxical response. Direct PCR sequencing of Galphas disclosed a mutant allele and a normal allele only at codon 201 in 4 of 8 tumors. The paradoxical response to TRH was observed in 2 of 4 patients without the mutation, and 2 of 4 patients with the mutation. The hTRH-R gene expression of pituitary adenomas did not differ between the tumors without the mutation and those with mutation. Conclusion: This study suggests that the expression of TRH-R gene is not likely to be a main determinant for the paradoxical response of GH to TRH, and that Galphas mutation does not seem to suppress the gene expression of TRH-R in GH secreting adenoma.
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Índice:
WPRIM
Asunto principal:
Neoplasias Hipofisarias
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Acromegalia
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Codón
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ADN
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ARN Mensajero
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Adenoma
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Expresión Génica
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Reacción en Cadena de la Polimerasa
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Receptores de Hormona Liberadora de Tirotropina
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Proteínas de Unión al GTP
Límite:
Humans
Idioma:
Ko
Revista:
Journal of Korean Society of Endocrinology
Año:
1997
Tipo del documento:
Article