The expression of heat shock protein 70/90 in patients with atrophic gastritis or gastric cancer and its significance / 中华消化杂志

ABSTRACT

Objective To investigate the expression of heat shock protein(HSP)70 and HSP90 in patients with atrophic gastritis(AG)or gastric cancer(GC)and its significance.Methods One hundred and forty-one patients including 35 with superficial gastritis(CSG),66 with AG(miner in 21,medium in 30 and severe in 15)and 40 with GC were enrolled with mean age of 47.8,56.1 or 59.4 years,respectively.H.pylori positive patients were 12 with CSG and 28 with AG.The quantity analysis of HSP90 and HSP70 were detected by immunohistochemistry,whereas their mRNA and protein expressions were measured by Western bolt and real-time PCR,respectively.Results The mRNA expression of HSP70 was significantly higher in CSG(1.31±0.80)and AG(1.41±0.80)than that in GC(1.18±0.70,P＜0.05),but was significantly lower in miner(1.32±0.70)and medium(1.34±0.60)AG than that in severe AG(2.20±0.80,P＜0.05).The mRNA expression of HSP90 was kept on increasing in order of CSG(1.27±0.60),AG(1.53±0.80)and GC(1.84±0.70); or in order of miner(1.33±0.60),medium(1.47±0.90)and severe(2.75±0.70)(P＜0.05).The patients infected with H.pylori had higher expression of HSP90 compared with those without H. pylori infection (P＜0.05). Conclusion The synchronous increase of HSP90 and HSP70 indicates the aggravation of AG. When HSP90 is up-regulated and HSP70 is down-regulatied, it may predict the occurrence of cancer.