The effect of respiratory syncytial virus infection on neutrophil adherence to airway epithelial cells / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
; : 50-61, 1999.
Article
en Ko
| WPRIM
| ID: wpr-38132
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Respiratory syncytial virus (RSV) is the most common cause of lower respiratory infection in infants and young children, but the pathogenesis of RSV-induced inflammation is not well defined. MATERIAL AND METHOD: In order to examine the potential interactions between virus-infected airway epithelial cells and neutrophils, we studied the ability of neutrophils to adhere to yirus-infected airway epithelial cell monolayers by myeloperoxidase assay. Also we measured the ability of airway epithelial cells to secrete interleukin-8(IL-8) and inter-cellular adhesion molecule-1(ICAM-1) in virus-infected airway epithelial cell cultures by enzyme-linked immunosorbent assay(ELISA). The degree of IL-8 and ICAM-1 gene expression in the RSV-infected BEAS-2B cell cultures were analyzed by reverse transcription-polymerase chain reaction(RT-PCR). RESULTS: The RSV-infected BEAS-2B cell resulted in significantly enhanced level of neutrophil adherence compared to the uninfected control(p (0.001). IL-8 and ICAM-1 production significantly increased by RSV infection(p<0.05). There was a significant positive correlation between neutrophil adherence and IL-8 level(r=0.73, p=0.002), and ICAM-1 level (r=0.843, p=0.001) in RSV-infected cells. The degree of both IL-8 and ICAM-1 mRNA expression increased in the RSV-infected cells compared with the uninfected ones. CONCLUSION: These results suggest that RSV infection significantly enhances the production of IL-8 and ICAM-1 in airway epithelial cells which then results in increased neutrophil adherence.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Virus Sincitiales Respiratorios
/
ARN Mensajero
/
Expresión Génica
/
Interleucina-8
/
Peroxidasa
/
Molécula 1 de Adhesión Intercelular
/
Técnicas de Cultivo de Célula
/
Células Epiteliales
/
Inflamación
/
Neutrófilos
Límite:
Child
/
Humans
/
Infant
Idioma:
Ko
Revista:
Journal of Asthma, Allergy and Clinical Immunology
Año:
1999
Tipo del documento:
Article