The influence of inflammatory cytokines to the expression of pigment epithelium-derived factor / 中华眼底病杂志

ABSTRACT

Objective To evaluate the effects of inflammatory cytokines,including tumor necrosisfactor TNF-α and interleukins(IL-6 and IL-8),to the expression of pigment epithelium-derived factor(PEDF)in human retinal pigment epithelium(RPE)cells.Method Cuhured primary human RPE cellswere treated with 20,2,0.2,and 0.02 ng/ml of TNF-α,IL-6 and IL-8 separately.The levels of PEDFexpression were determined by Western blot of the supernant after 6,1 2,24 and 48 hours of culture.Results PEDF secretion of RPE cells was inhibited by TNF-α,IL-6 and IL-8 in a time-and dose-dependent fashion.Compared with the controls,the expression of PEDF decreased significantly in 0.02ng/ml and 6 hours group(F=7.14,P<0.05),2.00 ng/ml and 48 hours group(F=14.05,P<0.01),and 20.00 ng/ml and 24 hours group(F=11.53,P<0.01).TNF-α was the most strength inhibitor(F=14,P<0.01).Conclusion TNF-α,IL-6,and IL-8 could suppress the expression of PEDF in thecultured human RPE cells.