Clinical laboratory-base surveillance of extensively drug-resistant tuberculosis / 中华检验医学杂志

ABSTRACT

Objective To evaluate the first-line and second-line anti-tuberculosis drugs resistance of clinical Mycobacterium tuberculosis ( M. TB) isolates and the prevalence of Extensively Drug-Resistant Tuberculosis (XDR-TB) in hospital-base TB patients in Shandong province, China. Methods Absolute concentration method based on egg medium were examined for drug-resistance of M. TB strains to 4 first-line anti-tuberculosis drugs (INH, RFP, SM, and EMB) and 5 second-line drugs (FQ,CPM,AMK,and PAS).Results 1021 isolates were obtained from clinical TB patients at Shandong Provincial Chest Hospital during November 2004 to April 2007. Of those, 32 strains ( 3.1% ) were identified to Mycobacterium other than tuberculosis. Among 989 M. TB complexe isolates, the general resistance rate to first-line drugs were 32. 3% (95% CI28.7% - 35.8% ), 107 isolates ( 10. 8% ) were found to be MDR-TB. According to the revised Global XDR-TB Task Force definition, a total of 20( 18. 7% ) MDR-TB patients met criteria for XDR-TB. All XDR-TB patients were previously treated pulmonary tuberculosis cases. Conclusions The prevalence of resistant to second-line anti-tuberculosis drugs in Mycobacterium tuberculosis was very serious, and high prevalence of XDR-TB in hospital-base patients was found in high-level The study showed that it is important to successively survey the anti-tuberculosis drug resistance in clinical laboratory.