Screening of antigenic epitope of neutrophil-activating protein of Helicobacter pylori by phage display peptide Hbrary / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 357-362, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-383940
ABSTRACT
Objective To identify epitope of neutrophil-activating protein(NAP) of Helicobacter pylori(Hp).Methods Using the mouse monoclonal antibodies against NAP as selective molecular and immunoscrcening phage-display random 7-peptides library.The positive clones were sequenced and analyzed.Phage clones were chosen to immunize mice and to evaluate the potential of phagotopes as effective vaccines.Results One mimotope(FAHLATQ)showed a good match with the NAP at 140-143 AA(AHLA)and the serum of mice induced by the phage clone clearly recognized NAP.Conclusion This study suggests thatthe antigenic epitope could be mapped through screening the phage-display peptide library with monoclonalantibody and a mimotopo of NAP providing an ahernative approach for the diagnosis and development of avaccine for Hp.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Microbiology and Immunology
Año:
2008
Tipo del documento:
Artículo
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