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Analysis of HPV-other Samples by Performing HPV DNA Sequencing
Korean Journal of Pathology ; : 250-253, 2009.
Artículo en Inglés | WPRIM | ID: wpr-38537
ABSTRACT

BACKGROUND:

HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.

METHODS:

For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high-risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV-other samples of 209 patients who subsequently underwent cervical biopsy.

RESULTS:

For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.

Conclusion:

HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papillomaviridae / Biopsia / ADN / Cuello del Útero / Quimera / Análisis de Secuencia de ADN / Análisis de Secuencia por Matrices de Oligonucleótidos Límite: Femenino / Humanos Idioma: Inglés Revista: Korean Journal of Pathology Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papillomaviridae / Biopsia / ADN / Cuello del Útero / Quimera / Análisis de Secuencia de ADN / Análisis de Secuencia por Matrices de Oligonucleótidos Límite: Femenino / Humanos Idioma: Inglés Revista: Korean Journal of Pathology Año: 2009 Tipo del documento: Artículo