Expression change of hepatic gelsolin and potential effect of Xuebijing injection in septic rats / 中华创伤杂志

ABSTRACT

Objective To investigate the expression changes of hepatic gelsolin (GSN) and the potential effect of traditional Chinese medicine Xuebijing injection in septic rats. Methods A total of 104 male Wistar rats were randomly divided into four groups, ie, normal control group (n = 8), sham operation group (n = 32), cecal ligation and puncture group (CLP group, n = 32) and Xuebijing treatment group (n = 32). The sepsis animal models in the last two groups were developed by CLP. In Xuebijing treatment group, animals were treated intravenously with Xuebijing injection (4 ml/kg) at 2, 12, 24,36, 48 and 60 hours after operation, respectively. Animals were sacrificed respectively at 6, 12, 24, and 72 hours after operation to harvest blood and hepatic tissue samples. Markers of liver function, contents of GSN and Gc globulin and expression of GSN mRNA in the liver tissues were detected. Results Compared with normal control group and sham operation group, hepatic GSN content was decreased significantly at various intervals in CLP group (P ＜ 0. 05), which reached the lowest value of (1.42 ±0.50) ng/mg at 12 hours. Meanwhile, expression of GSN mRNA in the liver was markedly enhanced at six hours after CLP and remained a high value until 72 hours, compared with the sham operation group (1.203±0.053 vs 1.030 ±0.084, P ＜0.05). Similarly, the hepatic Gc globulin contents was increased in the CLP group compared with the normal control group and sham operation group (P ＜ 0.05).After treatment with Xuebijing injection, the hepatic GSN contents at 6-72 hours was increased gradually while serum ALT and AST levels at 6, 12 and 24 hours were reduced significantly in comparison to the CLP group (P ＜ 0.05). Nevertheless, treatment with Xuebijing injection showed no marked effect on hepatic GSN mRNA expression and Gc globulin release (P ＞ 0.05). Conclusions Hepatic GSN contents are persistently reduced in the development of sepsis and treatment with Xuebijing injection can markedly attenuate acute liver injury through increasing GSN contents in severe sepsis.