Adaptive experiment of VNUKOVO-32 vcell culture rabies vaccine strain in cell lines
Journal of Preventive Medicine
;
: 128-132, 2005.
Artículo
en Vietnamita
| WPRIM
| ID: wpr-3888
ABSTRACT
Vnukovo-32 (cell culture rabies virus vaccine strain) was propagated experimentally in primary hamster kidney cell (PHKC), primary Chick embryo fibroblast cell (CEF) and human diploid cell (WI-38). These 3 cell lines were infected with Vnukovo-32 by the same mount of infection (MOl) - lLD50/l000 cells. The result showed that the Vnukovo-32 was adapted on all experimental cell lines, met the criteria of master seed (the titer ranging 6.4-7.5 log LD50/ml), and reached the highest titer on the 7th - 9th day postinfection in three cell groups. Moreover, the highest titer of Vnukovo-32 was met in human diploid cell (WI-38), then in PHCK, and the lowest was in CEF. From these results, it is suitable to use WI-38 to propagate Vnukovo-32 for master and working seed. The procedure for propagation of Vnukovo-32 is set up.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Vacunas Antirrábicas
/
Células
Idioma:
Vietnamita
Revista:
Journal of Preventive Medicine
Año:
2005
Tipo del documento:
Artículo
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