Effect of CXCL16 on the biological behavior of human pancreatic cancer cell line PANC1 / 中华胰腺病杂志

ABSTRACT

Objective To investigate the effect of CXCL16 on the biological behavior of human panereatic cancel cells PANC1.Methods Exponentially growing PANCI cells was exposed exposed to different concentration of rhCXCL16(50,100,200 mg/ml)and CXCL16 antibody for4 h,and PANC1 without rhCLCL16 Ireatment was used as the control.The proliferation was determined by MTT method,adhesion rate was determined by Mqrtigel matrix,invasion and migration of PANC1 cells were assayed by Transwell chamber.Results After 100 ng/ml rhCXCL16 treatment,proliferation,adhesion rate,invasion and migration of PANC1 eels were 0.264±0.021.991.4± 8.6)%,1.246±0.216,1.361± 0.276,respectively;and the adhesion rate.invasion and migration were significantly higher than(20.6±3.2)%,0.259±0.013,0.199±0.008 in the control group(P<0.01).and without significant effect on proliferation.After 200 ng/ml rhCXCL16 treatment,proliferation,adhesion rate,invasion and migration of PANC1 cells were further improved.Conclusions rhCXCL16 could enhance the ability of adhesion,invasion and migration of PANC1 cells.