Co-circulation of Two Genotypes of Hepatitis A Virus from Sporadic Cases in Northeastern Area of Seoul, Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 371-377, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-39343
ABSTRACT
BACKGROUND:
In previous studies, most hepatitis A virus (HAV) isolates had been genotype IA in Korea. Recently, a small number of different genotypes were reported with an upsurge of acute hepatitis by HAV. We investigated the distribution of HAV genotypes.METHODS:
RNA was extracted from anti-HAV IgM positive sera which were collected from March 2007 to February 2008 at a tertiary care hospital in Northeastern Seoul, Korea. Nested reverse transcription (RT)-PCR and direct sequencing for VP1/P2A region of the HAV were performed.RESULTS:
A total of 699 cases with suspected acute hepatitis were tested for anti-HAV IgM, and positive results were obtained in 56 sera (8.0%), which were collected 2 to 15 days (median, 7 days)after the onset of symptoms. Of the 56 seropositive samples, 52 (92.9%) were positive for HAV RNA, among which 28 isolates (53.8%) belonged to genotype IA and the remaining 24 (46.2%) belonged to genotype IIIA. Both IA and IIIA genotypes were isolated from 6-7 neighboring administrative districts throughout the year without geographic or seasonal restrictions.CONCLUSIONS:
Co-circulation of two distinct HAV genotypes (IA and IIIA) was observed from the northeastern Seoul for the year studied.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Inmunoglobulina M
/
ARN Viral
/
Datos de Secuencia Molecular
/
Proteínas Estructurales Virales
/
Secuencia de Aminoácidos
/
Análisis de Secuencia de ADN
/
Virus de la Hepatitis A Humana
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Genotipo
Límite:
Adolescente
/
Adulto
/
Niño
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Año:
2008
Tipo del documento:
Artículo
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