Helicobacter pylori Urease Activity is Influenced by Ferric Uptake Regulator
Yonsei Medical Journal
;
: 39-44, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-39512
ABSTRACT
PURPOSE:
The role of the Ferric Uptake Regulator (FUR) in the acid resistance of Helicobacter pylori (H. pylori) has been thought to be independent of urease. However, we demonstrated in this study that Fur influences urease activity. MATERIALS ANDMETHODS:
A fur knockout mutant of H. pylori was constructed by replacing the Fur gene with a kanamycin resistant marker gene. The wild-type H. pylori and fur mutant were compared for survival. The integrity of the inner membrane of the bacteria was evaluated by confocal microscopy using membrane-permeant and -impermeant fluorescent DNA probes. Urease activity of intact H. pylori was measured between pH 3 and 8. Real time PCR of both strains was performed for urease genes including ureI, ureE, ureF, ureG, and ureH.RESULTS:
The fur deletion affected the survival of H. pylori at pH 4. The urease activity curve of the intact fur mutant showed the same shape as the wild-type but was 3-fold lower than the wild-type at a pH of less than 5. Real time PCR revealed that the expression of all genes was consistently down-regulated in the fur mutant.CONCLUSION:
The results of this study showed that fur appears to be involved in acid resistant H. pylori urease activity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Represoras
/
Proteínas Bacterianas
/
Ureasa
/
Helicobacter pylori
/
Microscopía Confocal
/
Concentración de Iones de Hidrógeno
/
Modelos Biológicos
/
Mutación
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2010
Tipo del documento:
Artículo
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