Effects of quercetin and quercetin in combination with cisplatin on adhesion, migration and invasion of HeLa cells / 中华妇产科杂志

ABSTRACT

Objective To explore the effects of quercetin and quercetin in combination with cisplatin on adhesion, migration and invasion of HeLa cells. Methods Adhesion, migration and invasion of HeLa cells treated with quercetin and quercetin in combination with cisplatin were measured by adhesion assay, wound healing assay, and transwell chamber method respectively. Results The results showed that quercetin and quercetin in combination with cisplatin could inhibit adhesion, migration and invasion of HeLa cells. The adhesive ratio, migration rate and the invasiveness were negatively proportional to concentration of quercetin and quercetin in combination with cisplatin. With increasing concentration of quercetin from 20 to 80 μmol/L, the adhesive ratio decreased from ( 82. 2±1. 5 ) % to ( 48. 4±1. 1 ) % ; the migration rote decreased from (7.26±0. 20) μm/h to (3.78±0. 64) μm/h; the invasiveness decreased from ( 124. 3± 1. 5) piece to (90. 7±2. 1 ) piece(P <0. 05). In quercetin and quereetin in combination with 10 μmol/L cisplatin treatment group, with quercetin concentration increasing from 20 to 80 μmol/L, the adhesive ratio decreased from (42. 6±1. 2) % to ( 27. 5±1. 7 ) %, the migration rate decreased from ( 2. 20±0. 33) μm/h to (0.72±0. 19) μm/h and the invasiveness decreased from (78.7±2.5) piece to (44.0±4.0) piece (P < 0. 05 ). Compared with the quercetin groups, querectin in combination with cisplatin groups had significantly higher inhibitory effects ( P < 0. 05 ). Conclusions Quercetin and quercetin in combination with cisplatin can inhibit adhesion and migration and invasion of HeLa cells. Quercetin can enhance the inhibitory effect of cisplatin on HeLa cell adhesion, migration and invasion.