Influence of TAMs on monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 in NSCLC / 中国基层医药

ABSTRACT

Objective To observe cocultured TAMs cell U937 and NSCLC cell A549 effecting on MCP-1, MIP-1 expression under different conditions in vitro. Methods The cell UM937 of different concentrations (0,1,2,4,8×105/ml) was respectively selected to coculture with the cell A549 0f certain concentration (1.0×105/ml) for 24 hours, in situ hybridization was carried out to detect MCP-1, MIP-1mRNA positive expressions in cell A549 of every concentration group. Under the condition that both concentrations of cell A549 and cell U937 are l.0×105/ml, the same method was used to measure MCP-1, MIP-1mRNA positive expressions in cell A549 in different periods (4h, 8h, 16h, 32h, 48h). Results The positive expression ratios of MCP-1, MIP-1mRNA in cell A549 significantly increased along with addition of the cell U937 concentration and delay of the cocultural time, apparently higher than that of primary cocultured group that the cell U937 was 0×105/ml and that of contrast group at every period (all P<0.05). Conclusion The mutual interaction between TAMs and NSCLC cells has closely positive correlation with MCP-1, MIP-1mRNA positive expressions in cell A549, and depends on cocultural concentration and time.