Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1843-1846, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-402544
ABSTRACT
BACKGROUND:
Human amniotic epithelial cells(AECs)are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair.Currently,marking and tracing of human AECs remains rarely reported.OBJECTIVE:
To explore the efficiency of adeno-associated virus(AAV)vector-medicated green fluorescent protein(GFP)on in vitro cultured human AECs transfection.METHODS:
Human amnion samples were harvested and trypsinized to isolate human AECs.The AECs were transfected with AAV-GFP,and the transfection efficiency was detected.RESULTS ANDCONCLUSION:
Human AECs were successfully primary cultured and passaged in vitro.AAV-GFP-transfected AECs stably and highly expressed GFP,with a transfection efficiency of 58%.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2010
Tipo del documento:
Artículo
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