Bioinformatic analysis of streptococcus mutans thrS gene and construction of homologous recombinant plasmids / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1231-1234, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-403036
ABSTRACT
BACKGROUND:
Streptococcus mutans (S. mutans) is generally considered to be the principal aetiological agent for dental caries, thrS gene may relate to the virulence of S. mutans involved in the adherence, acidogenicity and acidodurance.OBJECTIVE:
To investigate the conservation status of the thrS gene of S. mutans and to construct the homologous recombinant plasmid.METHODS:
Southern Blot was used to analyze the distdbuUon of thrS gene in S. mutans. The upstream and downstream sequences of thrS gene were cloned respectively into multiple cloning sites of suicide plasmid pFW5 to construct the recombinant plasmid,RESULTS ANDCONCLUSION:
The thrS gene was conserved in 6 strains of S. mutans in this test. By PCR analysis and enzyme digesting, it was confirmed that S. mutans thrS gene homologous recombinant plasmid was successfully constructed,which can be used in future research of construction of thrS -negative mutans of S. mutans strain UA159.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2010
Tipo del documento:
Artículo
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