Studies on the relationship between pulmonary function changes with Foxp3,TGF-β1/Smads signal transduction pathway in adjuvant arthritis rats / 中国免疫学杂志

ABSTRACT

Objective:To observe the changes of lung index,lung function,regulatory T cells and Foxp3,TGF-β1,Smad3,Smad7 protein expression in adjuvant arthritis(AA) rats,and to investigate the possible mechanism of Foxp3 and TGF-β/Smads signal transduction pathway in reducing lung function.Methods: 24 Wistar rats were randomly divided into normal control group and model group,12 rats in each group.The rats were injected with 0.1 ml Freunds'complete adjuvant to induce inflammation at the part of their rightback foot in model group.On the 48~(th) day post-inflammatory,the swelling degree of toe and arthritis index(AI) were observed.Lung index of the rats was calculated,and pulmonary function of the rats was detected.The percentage of regulatory T cells was determined,and pathological changes of the lungs was observed by HE staining.Foxp3,TGF-β1,Smad3,Smad7 protein expression in the lungs of the two groups of rats was examined by immunohistochemistry.Results:①Compared with those of the normal,the swelling degree of toes,AI,and the lung index were increased.The average peak expiratory flow in 1 second (FEV1/FVC),alveolitis points,TGF-β1 and Smad3 protein expression was significantly higher in the AA model group rats(P＜0.01).Forced vital capacity (FVC),25% of vital capacity of the peak expiratory flow (FEF25),50% of vital capacity of the peak expiratory flow (FEF50),75% of vital capacity of the peak expiratory flow (FEF75),maximum mid-expiratory flow (MMF),forced maximal expiratory flow (PEF),pulmonary dynamic compliance (Cldyn),the percentage of CD4~+ T cell,CD25~+ T cell,CD4~+ CD25+~ T cell,Foxp3 and Smad7 protein expression was obviously lower in the AA model group rats(P＜0.01).②FEF50,Cldyn and MMF were negative correlated with the swelling degree of toes,pulmonary coefficient and TGF-β1.FEF50,FEF75,MMF and FEV1/FVC were positiyely correlated with the arthritis index,pulmonary coefficient and Foxp3(P＜0.05 or P＜0.01).Conclusion:The decline in lung function in AA rats.Tip-induced may be sustained after the inflammation,and the development of cause inflammation,to chronic inflammatory response leading to lung injury.Simultaneously,the decline in lung function and Foxp3.Smad3,Smad7,TGF-β1 protein expression was associated.Description transcription factor Foxp3 and TGF-β1/Smads signal transducation pathway may participate in the mechanism.