Screening of binding proteins of HMGB1 promoter by phage display technique / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 28-31, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-404160
ABSTRACT
AIM:
To screen the binding proteins to HMGB1 promoter by phage display technique.METHODS:
HMGB1 promoter was incubated with phage display library. Unbound phages were eluted and phages bound to HMGB1 promoter were amplified. Twenty individual clones were randomly selected and identified by enzyme-linked immunosorbent assay (ELISA). Positive clones were characterized by DNA sequencing and the sequences were subjected for computer analysis.RESULTS:
Positive phages binding to HMGB1 promoter were enriched after 4 rounds of biopanning. Twenty phage clones were selected and eleven clones of which were identified to bind specifically to HMGB1 promoter. The sequences in full length were obtained and searched for homologous sequences from GenBank. Altogether eight coding sequences were obtained, six of which were known proteins including activator protein-1(AP-1) and two of which were uncharacterized ones.CONCLUSION:
Several proteins were obtained that bind specifically with HMGB1 promoter. The results will be useful for further studying the expression and regulation mechanism of HMGB1.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
2010
Tipo del documento:
Artículo
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