Effects of lycium barbarum polysaccharide on retinal morphometry and function of retinal photic injury in rats / 眼科

ABSTRACT

Objective To investigate the functional protective effect of lycium barbarum polysaccharide (LBP) against light-in-duced retinal degeneration. Design experimental studies. Participants sixty SD rats were divided randomly into 6 groups. Methods Sprague-Dawley rats were exposed for 24 hours to 2000-lux cool white fluorescent light after treatment with LBP. Scotopic flash elec-troretinogram (ERG) were recorded before and on day D1, D6 and D14 after light exposure. The b-wave amplitude was statistic. In addi-tion, rats from each group were killed for retinal morphometric analyses. Main outcome Measure The amplitudes of b-wave of scotopic flash ERG, the histopathological changes. Results In the untreated group, light exposure caused collapse of the b-wave sensitivity curves. Bmax was reduced by 29.97% at D1 without subsequent recovery. Histopathology of the temporal superior central retinas of rats showed outer segment disorganization and shortening, the inner segments swelled and vacuolated, the outer nuclear layer thicknesses decreased markedly with subsequent recovery. In the treated groups, light exposure had a weaker effect on sensitivity curves. The values of b-wave amplitude were significantly increased than those in the exposed-untreated group (P=0.005). Retinal morphometry was pre-served. Conclusion LBP afford functional protection against light-induced retinal damage. (Ophthalmol CHN, 2009, 18 229-233)