Clinical application of serum tissue polypeptide-specific antigen detection in diagnosis of alcoholic hepatitis / 国际检验医学杂志

ABSTRACT

Objective To investigate the role of serum tissue polypeptide-specific antigen (TPS)detection in the evaluation of hepatocyte apoptosis in patients with alcoholic hepatitis and to further evaluate its application in the differential diagnosis of alcoholic hepatitis and fatty liver. Methods The automatic biochemistry analyzer was employed to detect common markers for liver injury (AST,ALT, GGT and bilirubin) ; electrochemiluminescence assay was performed to measure serum TPS level; immunohistochemistry staining was done to evaluate M30 immunoreactivity in hepatic tissue biopsy specimens and the apoptotic score was acquired. Then the correlations were analyzed between various indices and the apoptotic scores. Results The serum TPS level was positively correlative to the hepatocyte apoptotic score in alcoholic hepatitis group; and their correlation coefficient was the largest among various liver functional indices and the apoptotic scores. The correlation coefficient between TPS level and apoptotic score was significantly higher in alcoholic hepatitis group than that in fatty liver group (P＜0.05). Conclusion Serum TPS may be used as a good hepatocyte apoptosis marker for alcoholic hepatitis, which contributes to the differential diagnosis of alcoholic hepatitis and fatty liver.