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Characterization of Recombinant Human Necrosis Factor-α ReceptorⅡ:IgG Fc Fusion Protein by Nano Ultra-high Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry Tandem Mass Spectrometry / 分析化学
Chinese Journal of Analytical Chemistry ; (12): 1025-1028, 2009.
Artículo en Chino | WPRIM | ID: wpr-406239
ABSTRACT
The Nano ultra-high performance liquid chromatography-electrospray ionization mass spectrometry tandem mass spectrometry(UPLC-ESI-MS/MS) was used to characterize the primary protein. Samples were digested by trypsin, followed by liquid chromatography-tandem mass spectrometry analysis and database search. Five peptides matched with tumor necrosis factor receptor and seven peptides matched with human IgG1 fragment of the Fc. Further analysis demonstrated that seven peptides all matched with the IgG1 Fc but only partly peptides matched with the other subtypes. RhTNFRFc is fused by IgG1 Fc but not other subtypes.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: Chinese Journal of Analytical Chemistry Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: Chinese Journal of Analytical Chemistry Año: 2009 Tipo del documento: Artículo