Effects of recombinded resistin on pyruvate carboxylase mRNA expression and activity in culture bovine hepatocyte in vitro / 中国兽医学报
Chinese Journal of Veterinary Science
;
(12): 924-927, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-406336
ABSTRACT
A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte.Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L.Abundance of PC mRNA in bovine hepatocyte,which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluorescence quantitative RT-PCR,and activity of PC was determined by colourimetry.The results showed that bovine recombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Veterinary Science
Año:
2009
Tipo del documento:
Artículo
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