Determination of the contents of mental elements and total fiavone in Scutellaria baicalensis Georgi

Junmin WANG; Liqin HAN; Shunfu DONG

ABSTRACT

BACKGROUND:

Traditional Chinese medicine (TCM) has clear efficacy and fewer side effects, because of its own pharmacological action, as well as the trace elements in it.

OBJECTIVE:

To determine the contents of metal elements and their total flavone in Scutellaria baicalensis Georgi (SBG).

DESIGN:

Repeated measurement trial.

SETTING:

Jilin Medical College, Affiliated Hospital of Jilin Medical College.MATERIALS SBG was bought on the market. Rutoside sample(F 20050930, Sinopharm Group Chemical Reagent Co., Ltd.).

METHODS:

The experiment was carried out in the Department of Trace Element in Chinese Herbal Medicine, Jilin Medical College from April to July in 2006. SBG samples were dried and powdered then digested with nitric acid-perchloric acid wet method. And 1.000 0 g was weighed accurately, 3 portions were used for each kind of sample, placed in 25 mL coni cal flask, 10 mL concentrated HNO3 was added, then sealed for overnight.10 mL concentrated HNO3 and 5 mL HClO4 were added respectively on the next day, and slowly heated on the electric hot plate; when only about 1 mL left, 20 mL concentrated HNO3 and 5 mL HC1O4 were added for continuous digestion till the solution was colorless and clearing and almost dried. It was diluted and dissolved with 30 g/L nitric acid, then transferred to 50 mL volumetric flask, evenly mixed for measurement. Atomic absorption spectrophotometer was used to detect macroelement Na, Ca, Mg, K and trace element Fe, Mn, Cu, Zn. Alcohol extraction method was applied to extract the total flavone of SBG while UV spectrophotometry for the content of total flavone.MAIN OUTCOME

MEASURES:

The contents of metal elements and their total flavone.

RESULTS:

The contents of metal elements and total flavone in SBG were detected as follows K (1 098.516 8±2.731 9) μg/g; Ca (6 561.552 6±78.716 7) μg/g; Na (9 530.491 2±44.176 6) μg/g; Mg (6 555.442 8±36.860 3) μg/g; Fe (512.075 0±5.135 9) μg/g; Cu (2.905 0±0.134 2) μg/g;Zn (30.081 0±0.740 0) μg/g; Mn (12.409 2±0.247 7) μg/g; Total flavone(17.496 2±2.134 4) mg/g.

CONCLUSION:

Metal element and their total flavone are rich in SBG.