The preliminary study on the bone marrow-derived mesenchymal stem cells migrating toward glioma / 中国神经精神疾病杂志

ABSTRACT

Background Bone marrow-derived mesenchymal stem cells (MSCs) have been reported to have the ability of migrating after transplantation into rat model of traumatic brain injury. In this study, MSCs'characteristic of tropism for intracranial glioma was explored following being labeled and transplanted into brain and blood of gliomabearing rats.Methods Cellulae medullares were cultured to get pure MSCs. The cell surface antigen and cell cycle of MSCs were detected to confirm their identity by flow cytometry. MSCs were marked with BrdU and then were injected into contralateral brain or collateral internal carotid artery of rats bearing glioma. 2 weeks later, the brains were resected and pathological sections were made. Immunohistochemistry and immunofluorescence technology were performed to detect MSCs.Results MSCs displayed extensive tropism for intracranial glioma. MSCs which were injected in the contralateral brain of the glioma scattered densely in the juncture between brain tissue and tumor and there were a few MSCs in the glioma; MSCs which were injected into the collateral internal carotid artery scattered widespread in the glioma and there were a few MSCs in the juncture area.Conclusions MSCs have the ability to migrating toward glioma and penetrating blood brain barrier. MSCs may be ideal gene therapy vehicles against glioma.