Experiment in effect of jiangtang zhongyao No.1 on islet cell secretion in vitro

Ying LIU; Zhenfang LIU

ABSTRACT

BACKGROUND:

On bases of preceding study on jiangtang zhongyao No.1 (glucose-reducing No. 1), the experiment was performed in its effect on promotion of insulin secretion of islet cells in vitro.

OBJECTIVE:

To explore the mechanism of glucose-reducing No.1 so as to provide the scientific evidence for clinical experiment.

DESIGN:

Neonatal porcine islet cells were collected for generative culture to observe the effect of glucose-reducing No.1 on insulin secretion of islet cells cultured in vitro.

SETTING:

Department of Pathology of Shandong Province Hospital and Neurological Department of Shangdong Province Hospital of Shandong University.MATERIALS The experiment was performed in Research Room of Chinese Herbal Pharmacology in Shandong Academy of Medical Sciences, in which, American neonatal pig was selected as the object.

METHODS:

The obtained neonatal porcine islet cells were randomized into 5 groups, 3 bottles in each group. In culture solution group, the culture solution of equal volume was added. In daonil group, daonil 4 nmol/L was added and in glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL groups, the filter solution of glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL were added successively. The samples in each group were incubated in culture box for 3 hours, and then the upper clear solution was collected to assay insulin content with radioimmunoassay (RIA).MAIN OUTCOME

MEASURES:

Comparison of active components of islet cells in each group.

RESULTS:

In basic culture solution, insulin levels were not different significantly among groups. The release amounts in glucose-reducing No. 10.2 mL, 0.15 mL and 0.1 mL groups were increased compared with insulin content in basic culture solution [(784.72±81.25), (462.25 ±14.91);(711.73±91.46), (467.62±15.56); (679.40±78.84), (475.82±13.33) mU/L],and multiple proportion relationship was not obvious with dosage of Chinese herb. In daonil group, insulin secretion was increased significantly [(857.96±77.08), (470.58±15.43) mU/L].

CONCLUSION:

Jiangtang zhongyao No.1 simulates islet cells to increase significantly the secreted amount of insulin so that the glucose is well reduced.