Experimental study of gut absorptive capacity after trauma detected by D-xylose content test with micromethod / 中国组织工程研究

ABSTRACT

Aim To establish a rapid and sensitive diagnostic markerfor adjusting gut absorptive capacity after trauma. Methods A mi-cromethod for D-xylose test with phloroglucinal was established according toEberts with modification. The standard curves were repeated for fivetimes. Then the blood contents of fasting and orally taking 0. 5 g/kgD-xylose at 2nd and 4th hour were measured and the blood D-xylose con-tents of seven refilling rats with ischemia of intestine were mea-sured. Results The maximal absorption spectra was read at 554 nm byscanming of DU-7 Beckman. Good linearity of the D-xylose standard curvewas found with the range of 0 to 4 mmol/L(r=0. 9979 ±0. 0017) . Theblood content of rats at 2nd hour after orally taking D-xylose was ( 154 ± 6)mg/L amd that at 4th hour was decreased to (87 ± 11) mg/L. And theblood content at 2nd and 4th day after ischemic refilling was (162 ± 5)mg/L and (80 ± 8) mg/L respectively. The variation coefficients within andamong batches were 1.98% (n=6) and 3.10% (n=6), respective-ly. And D-xylose recovery rates were from 97.2% to 104. 3%. ConclusionMicromethod for D-xylose test appears to be simple, rapid and sensitive,and is an available index for estimating intestinal absorption after severetrauma.