Effect of genistein on c-myc mRNA expression induced by low density lipoprotein in endothelial cells / 中国新药与临床杂志
Chinese Journal of New Drugs and Clinical Remedies
;
(12): 5-8, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-410130
ABSTRACT
AIM:
To study the effect of genistein on c-myc mRNA expression induced by oxidized low density lipoprotein (ox-LDL) in human vascular endothelial cells (ECV304).METHODS:
LDL were isolated from healthy human plasma by gradient ultracentrifugation and oxidized by CuSO4. ECV304 cells were exposed to ox-LDL 200 mg*L-1 in the presence or absence of genistein 100 μmol*L-1 for 1, 2, and 4 h in vitro. Northern blot was employed to measure c-myc mRNA levels of ECV304.RESULTS:
In response to ox-LDL 200 mg*L-1, c-myc mRNA expression in ECV304 increased by 3 fold for 1h and 3.3 fold for 2 h and decreased below the control level at 4 h. Expressions of c-myc stimulated by ox-LDL in the presence of genistein 100 μmol*L-1 for 1 h and 2 h were separately 80 percent and 60 percent of that in the absence of genistein 100 μmol*L-1.CONCLUSION:
Genistein can effectively inhibit c-myc mRNA expression in ECV304 induced by ox-LDL.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of New Drugs and Clinical Remedies
Año:
2003
Tipo del documento:
Artículo
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