Effects of negative PTFE electrets on the apoptosis of fibroblast cells / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 409-411, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-410256
ABSTRACT
Objective:
To study the influence of negative electrets on apoptosis of fibroblast cells and to probe its mechanism.Methods:
Fibroblast cell were treated with -300, -500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope.Results:
Compared with control group, apoptosis cells increased from 0.5% to 10% (some even to 15%) after 24,48 and 72 h action of -300, -500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles (apoptotic bodies). The effect of negative electrets on apoptosis was in proportion to the time and electric field intensity.Conclusion:
Negative electrets can enhance apoptosis of fibroblast cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2001
Tipo del documento:
Artículo
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