The Mechanisms of Arsenic Trioxide-induced Apoptosis in Human Gastric Adenocarcinoma SGC7901 Cells / 中国药房

ABSTRACT

OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.