Protective effect of cardiomyopeptidin on cultured myocardial cells injured by anoxia-reoxygenation / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 481-484, 2001.
Article
en Zh
| WPRIM
| ID: wpr-410302
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WPRO
ABSTRACT
AIM: To study the protective effect of cardiomyopeptidin (CMP) attributed to polypeptide on cultured myocardial cells injured by anoxia-reoxygenation.METHODS: The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. The effect of CMP on myocardial ultrastructure was observed. [Ca2+]i was estimated with adherent cell analysis and sorting 570(ACAS 570) laser cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique. RESULTS: CMP could obviously improve the ultrastructure of myocardial cells and dose-dependently decrease [Ca2+]i and increase the lipid fluidity of cellular membrane, CMP also could markedly reduce the chromaticity value of pseudo-colour graphic model of Ca2+. CONCLUSION: Cardiomyopeptidin has an obvious protective effect on cultured myocardial cells injured by anoxia-reoxygenation, this may be related to its effect of decreasing [Ca2+]i and increasing lipid fluidity of cellular membrane.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Año:
2001
Tipo del documento:
Article