Monitoring the Process of Bovine Insulin Unfolding by Capillary Zone Electrophoresis / 分析化学
Chinese Journal of Analytical Chemistry
;
(12): 538-541, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-410362
ABSTRACT
Capillary zone electrophoresis (CZE) was used to monitor the whole unfolding process of bovine insulin resulted from the dithiothreitol (DTT) reduction. An off-line matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was employed to determine the molecular weight of the reaction products at the same time to confirm the observation results obtained by CZE. The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously. The results indicated that as an effective tool of monitoring the conformational change of protein,the method of CZE was simple,quick,sensitive and lower sample consumption.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Analytical Chemistry
Año:
2001
Tipo del documento:
Artículo
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