Human mesenchymal stem cells differentiate into neuron-like cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 385-387, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-410425
ABSTRACT
AIM:
To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS:
hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry.RESULTS:
hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express.CONCLUSION:
hMSC can be induced to differentiate into neurous.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
2001
Tipo del documento:
Artículo
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