Effects of DS201 on Proliferation of Pulmonary Artery Smooth Muscle Cell Induced by Hypoxia / 华中科技大学学报(医学版)

ABSTRACT

Immunocytochemistry, flowcytometry and nucleic acid in situ hybridization were used to observe the effect of sodium tanshinon Ⅱ-A silate (DS201) on proliferation of pulmonary artery smooth muscle cell (PASMC) induced by hypoxic endothelial cell condition medium (HECCM). The results showed that DS201 could obviously hinder PASMC to enter S phase from G0/G1 phase (P<0.05), and decrease the expression of PCNA and platelet-derived growth factor (PDGF)-A and B chain mRNA in PASMC respectively, (P<0.01). It was suggested that DS201 could probably inhibit the prolifertion of PASMC mediated by HECCM, through down-regulating the expression of PDGF-A and B chain gene.