The Gene Cloning, Expression and Bioactivity of the Human FKBP52 / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
;
(12): 236-239, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-411263
ABSTRACT
To obtain active hFKBP52 protein for screening novel neu rotrophic drugs. Semi-nested and overlap PCR and affinity chromatography were u sed. hFKBP52 gene was cloned successfully from human fetal brain cDNA library, a nd then highly expressed (about 30%) as fusion protein in pET28a(+) vector syste m. The recombinant protein was purified as one band on SDS-PAGE. The purified h FKBP52 showed peptidyl-prolyl cis-trans isomerase (PPIase) activity, simil ar to the wild type.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Progress in Biochemistry and Biophysics
Año:
2001
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS