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The effect of OX-LDL and simvastatin on PKC activity and cytosolic free Ca 2+ in cultured human monocytes / 中国药理学通报
Chinese Pharmacological Bulletin ; (12): 178-180, 2001.
Artículo en Chino | WPRIM | ID: wpr-411452
ABSTRACT
AIM To investigate the effect of OX-LDL and HMG- CoA reductase inhibitors simvastatin on PKC activity and cytosolic free Ca2+ in cultured human monocy tes. METHOD The activity of PKC was determined by its ability to tr ansfer phosphate from [32P]ATP to lysine-rich histone and cytosolic free calcium[Ca2+]i was measured by flow cytometric analysis loading with the Ca2+ dye fluo3/Am. RESULTS OX-LDL increased PKC tot al activity in a dose-dependent manner with phase peaking at 12 min, then decre ased slowly and maintained for at least 20 min, while OX-LDL induced biphasic [Ca2+]i responses including the rapid initial transient phase and the sustained phase. Removal of extracellular Ca2+ did not inhibit the rapid i nitial transient phase of OX-LDL-induced rise in [Ca2+]i,but abolish ed the sustained phase of [Ca2+]i response to OX-LDL. When simvastati n was added, the activity of PKC was markedly decreased and simvastatin did not impair the initial peak response to OX-LDL but significantly reduced the subseq uent plateau phase. CONCLUSION OX-LDL can significantly activate t he activity of PKC and elevate [Ca2+]i in monocytes. The rapid initial transient phase was the result of mobilization of [Ca2+]i from intrac ellular pool and sustained phase resulted from the influx of extracellular Ca 2+. The inhibition of PKC activity induced by simvastatin may be contribute to the changes of intracellular Ca2+.
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Pharmacological Bulletin Año: 2001 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Pharmacological Bulletin Año: 2001 Tipo del documento: Artículo